Voice 260603_195446 Rachel Meeting

Puheenvuorot

• Speaker 1 1:22
• Speaker 2 1:38
• Speaker 3 1:06:03

1. 1:22

   Luku 1: So for my presentation, I will write both PI's name or how to write. 300s · Speaker 1

   So for my presentation, I will write both PI's name or how to write. I'm still confused. For the presentation. Let me ask Jennifer tomorrow but I think I will be because they are just for paperwork. Should I write or didn't need to write? I…

2. 6:23

   Luku 2: Oh, okay. 303s · Speaker 1

   Oh, okay. Because I just want to add some aims here. I'm sorry. Okay, so what do you what do you think about those genes that you Well, the genes that you found specific present, the SNP specific present in the CM2. Yeah, how you can dig in…

3. 11:26

   Luku 3: MAGE -9 population provide a common B73 framework. 300s · Speaker 1

   MAGE -9 population provide a common B73 framework. So to study how natural effect complexity. So this is important because this is based upon B73. I think it's okay. So then I will introduce them about the Chasma because so we will I will i…

4. 16:27

   Luku 4: And then this is, we want to answer this question because the observation, which is CM22A has lower crossover. 300s · Speaker 1

   And then this is, we want to answer this question because the observation, which is CM22A has lower crossover. So we want to know which step limited that. So that doesn't really make, that makes sense a lot. this to answer this doesn't real…

5. 21:28

   Luku 5: Change. Well, so it's because. 300s · Speaker 2

   Change. Well, so it's because... I think modify is more... Modify. Yeah. Because, yeah, because the gene in the wild type, I mean, if we found some, if we found a gene that in CML228 make all this, that gene is commodified. This is a big co…

6. 26:28

   Luku 6: And we just have 20 or something. 304s · Speaker 1

   And we just have 20 or something. But in the Gamma tracks, if the little dim will miss, so you will miss a lot of signals. There are very few that are very bright. Okay, so Haytan should reduce class 1 formation in CML2A. So the next thing …

7. 31:33

   Luku 7: minor but yes yes Then we will introduce this, the fact that the DSP numbers can reduce crossover formation and the DMC standard deviation progression can also reduce crossover formation. 300s · Speaker 2

   minor but yes yes Then we will introduce this, the fact that the DSP numbers can reduce crossover formation and the DMC standard deviation progression can also reduce crossover formation. So that's why we also want to check and that is the …

8. 36:33

   Luku 8: uh global mean like there is no any specific part where here then is but then the the the the haters reduce almost half so now we have a for example 14 hated so we will see their pattern at that all the 14 are pattern yeah or at that they are parenting a mix so forth so I think I think you can probably for example I would I will think this can be In other line, 20 crossovers on 20 arms. 304s · Speaker 2

   uh global mean like there is no any specific part where here then is but then the the the the haters reduce almost half so now we have a for example 14 hated so we will see their pattern at that all the 14 are pattern yeah or at that they a…

9. 41:37

   Luku 9: order. Or how about you maybe just use the word. 303s · Speaker 1

   order. Or how about you maybe just use the word. Sometimes it's all about phrases. Maybe you can use... Well, let me say it first. you have now we have reduced crossover whether they reduce whether those crossovers still whether their distr…

10. 46:41

    Luku 10: sites are associated with low cg ch gemethization and open chromatin so i will explain this briefly and so this is already from this portrait literature so we have I use that figure because this can also be used for the M2. 300s · Speaker 2

    sites are associated with low cg ch gemethization and open chromatin so i will explain this briefly and so this is already from this portrait literature so we have I use that figure because this can also be used for the M2. So this is for p…

11. 51:42

    Luku 11: so that's why I normally use V. 299s · Speaker 1

    so that's why I normally use V. Yeah, but actually you can, I think. V for all the things. Oh, okay, okay, that's fine, okay. Well, yeah, but you can, you know, confidence it. Yeah, but I cannot do without. There is a model sport, a lot of …

12. 56:46

    Luku 12: Okay, so if there is a large structural variation, say a big deletion, that deletion is based on comparison between CM22A and B72A. 67s · Speaker 2

    Okay, so if there is a large structural variation, say a big deletion, that deletion is based on comparison between CM22A and B72A. And then that deletion normally will not cost crossover something, no, because deletions. the structural var…

13. 57:53

    Luku 13: cold spot I'm thinking this way so that is happening only in the hybrid yeah yeah yeah so I'm thinking in this way like we have a region here this region so like this one is B73 and we find that there is a crossover cold spot Okay, so what's the resolution you say? 291s · Speaker 1

    cold spot I'm thinking this way so that is happening only in the hybrid yeah yeah yeah so I'm thinking in this way like we have a region here this region so like this one is B73 and we find that there is a crossover cold spot Okay, so what'…

14. 1:03:10

    Luku 14: For each recombination, for each high breeze, they are 200. 145s · Speaker 1

    For each recombination, for each high breeze, they are 200. And then I believe they are breaking, they map those sites, they map those breaking, break point. They map those recombination, but not precisely, but they know where, how, where a…

15. 1:05:43

    Luku 15: So we can. 303s · Speaker 2

    So we can... Well, you already have enough end, so it's fine if you just remove that. You don't need to have three sub -end for each big end. That's too many. And then you all got questions like, oh, how many years you want to start it for …

16. 1:10:47

    Luku 16: the machine to make crossover those machine is made of protein and then the protein is different from B73. 130s · Speaker 1

    the machine to make crossover those machine is made of protein and then the protein is different from B73. So those protein is transacting on the site. So then those are the transacting factors. So you can say, oh, you know, you need someth…

17. 1:12:57

    Luku 17: This is called manipulation. 303s · Speaker 2

    This is called manipulation. And then, so this is, yeah, so you don't need to, you can connect, you can say, oh, those are the... This is one model. So it's, yeah, yeah. So, well, actually, there's a review talking about, you know, this typ…

18. 1:18:01

    Luku 18: the way. Okay. 127s · Speaker 1

    the way. Okay. So unless later you define what is this, what is this better. I mean, you define in the following slide. But... But it makes more sense that this came from wild type. I mean, this came from Imbra -line study, right? I mean, o…

19. 1:20:08

    Luku 19: there is a cease effect, then crossover distortion is. 302s · Speaker 3

    there is a cease effect, then crossover distortion is... Yeah, yeah. I think previously I mentioned cease and trance actually means the crossover formation mechanisms in Silva 2 -2 -8. The lower crossover is caused by cease or trance. In th…

20. 1:25:10

    Luku 20: is difficult and yeah those are yeah that that things actually you know come into my mind several times that the hybrid is difficult unless For example, maybe you can, we can look at DSP formation in hybrid number because crossover number reduces. 301s · Speaker 1

    is difficult and yeah those are yeah that that things actually you know come into my mind several times that the hybrid is difficult unless For example, maybe you can, we can look at DSP formation in hybrid number because crossover number r…

21. 1:30:12

    Luku 21: Because it's not totally independent. 301s · Speaker 1

    Because it's not totally independent. Okay, so the candidate gene are those 150? Yes. And then... After hyperotype validation, we have some genes. So first we have RNA, we will narrow down it, then we will use hepatotype approach to narrow …

22. 1:35:13

    Luku 22: kind of validation in f1 maybe that is feasible no no no the validations cannot be f1 because f1 restored so f1 is not the material for validation F1 will actually open another question like well what's going on or what would happen if the sequence is not and for example like okay so what what makes what's a double string break formation and then what like you can I mean not some validation like approve how it's restored or yeah okay but then how right if we just check Say we have 10 genes and then we test it by qPCR. 300s · Speaker 2

    kind of validation in f1 maybe that is feasible no no no the validations cannot be f1 because f1 restored so f1 is not the material for validation F1 will actually open another question like well what's going on or what would happen if the …

23. 1:40:14

    Luku 23: no, no, sorry. 204s · Speaker 1

    no, no, sorry. This one. So, yeah. So, I just want to see the wording of his name. Okay. okay let me yeah yeah so just I guess so just see the wording so if there's a any word to me okay so then I will put the exact wording just name no nee…