1 DNA Isolation and purification (mp3cut.net)

May 06, 2026 05:14 · 37:32 · English · Whisper Turbo · 2 speakers

یہ نقل ختم ہو جاتا ہے 22 دن. دائمی محفوظہ کے لئے بہتری →

Lecture Index

0:00

S… Speaker 2 (1 DNA Isolation and purification (mp3cut.net))

And there is a mechanism and process in the lab where we can actually get a specific nucleic acid from the cell. That nucleic acid at the beginning of the world is containing a cell plate in the center of the cell. And before we are going to get the data, we have every aspect of this cell, including the glyphosateic bond, phosphorizer bond, other phospholumids, and the cellular G3, which is present inside the cytoplasm as a protocol. And there is a specific...

0:32

S… Speaker 2 (1 DNA Isolation and purification (mp3cut.net))

mechanism, we have the first membrane first, the second membrane then isolate these membranes together with the help of these chill particles and after that the chilleting particles appear in the solution that particles will be now instead of this we are isolating that thing instead of this they contain some sort of debris and the contaminated molecules with the multiple problems and the remaining particular bud that will be the final and the purest form of some really present

1:33

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

This is a simple simple thing that you can read about the composition, structure, etc. Now, you have to know the location of DNA is where it is. I have told you that DNA is where it is. I have told you that DNA is where it is. You have to know that DNA is where it is. You have to know that DNA is where it is. You have to know that DNA is where it is. You have to know that DNA is where it is. You have to know that DNA is where it is. You have to know that DNA is where it is.

4:39

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

Why do we need to isolate or purify

6:29

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

and the DNA lab.

6:35

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

There are 5 diseases in the same way. There are 5 diseases in the same way. There are 5 diseases in the same way. There are 5 diseases in the same way. There are 5 diseases in the same way.

9:40

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

Monika, what is the reason why she is here?

10:00

S… Speaker 2 (1 DNA Isolation and purification (mp3cut.net))

for the process of mutation instead of we can go through the bio-imagining at a conditional basis. These are the learning objects which we have to study in today's class. After completing the lecture, you should be able to understand everything with the concept which is written on here. You have to answer everything related to the disease. These are the learning outcomes which you need to understand after completing the lecture.

10:50

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

DNA Isolation and DNA Purification are closely related problems. So what is the difference between Isolation and Purification? This is what happens when you remove it. Purification is that you remove the antibodies and you remove the antibodies. That means that we have pure DNA. Isolation is that? Isolation is that? Isolation is that? Isolation is that? But they are not extremely stable. They are the brother procedure.

11:58

S… Speaker 2 (1 DNA Isolation and purification (mp3cut.net))

But they are not extremely safe. They represent two stages of obtaining un-stabless DNA from the voluntary DNA. When you have to isolate things, you can understand that when you come in the class, there are many people who are isolated. They can be isolated, but instead of this, they have some misunderstood concepts.

12:38

S… Speaker 2 (1 DNA Isolation and purification (mp3cut.net))

When you isolate it, I had told you that we are only only in the debris. But the DNA itself is composed of multiple things. And we are not only in the sequencing, except that there is no protein, no nucleotide, etc. If you look at all these things, we are only in sequencing, that is a pure form, a purification. The gene of interest has been isolated with multiple processes, that is a purification. But whenever, I am just taking the DNA out of this case, that is an isolation.

13:12

S… Speaker 2 (1 DNA Isolation and purification (mp3cut.net))

Isolation from what? Every cellular debris has been isolated from the DNA. The DNA is separated and the cellular debris is not pure yet. To make it more purified. DNA isolation, this is the initial process of extracting DNA out of the cell other tissues. We have to take it in a pure form. We have to isolate and extract the cellular debris from the tissues.

14:05

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

I will tell you about the WC. Tell me about the tissues. From which we can isolate the DNA.

15:04

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

It removes the breaking, open the cell and freeing the DNA from the protein, membrane and other cellular cells. The first definition of the isolation is that the cellulant membrane is ruptured, cellulant membrane, protein etc etc etc etc etc etc etc etc etc

15:30

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

Instead of this, that DNA containing the impurities itself, okay? This is the beginning of this, when you have a cell in the cell, you have a cell in the cell, you have to remove the membrane from the cell, the nucleus of the whole nucleus of the whole DNA. Okay? Isolated to have a DNA, now you know, the whole structure is just here, that is Iacolic. But instead of this, there are also many impurities.

16:01

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

It is a pure form. When you remove the cellulose debris, you can remove the cellulose molecules. You can remove the cellulose molecules. When you remove the cellulose, you can remove the cellulose molecules. When you remove the cellulose, you can remove the cellulose molecules. When you remove the cellulose molecules, you can remove the cellulose molecules.

21:29

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

CT4 is nothing but a biomarker which leads at the time of the required immunity. CT4 is a cluster designation form.

22:08

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

These are the techniques.

22:29

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

from which you can purify the DNA from the purity.

25:00

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

I have a six-chemical reagent. One is a phenol and chloroform. On the other hand, I have a Proteinase K. Which one is more suitable for denaturing the cellular debris for acceleration of the DNA? Write down the reservoir. Both can be same for the lab professor. You can do both of it. The most important and more accurate one is a Proteinase K. Nowadays, which is happening in the lab professor.

25:48

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

The phenol are chloroforms denature the protein which is basically every cellular membrane is a metaphor. They separate the nucleic acid from the DNA. The same is using the SGS. What is SGS? Sodium, deuterium, sulfate, double salt, cellular debris. Number 2. Additional phenol chloroform centrifuge for the

26:31

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

If you have a solution, you will have a solution. That is the reason why you are getting the phenol chloroform. This is the reason why you are getting the phenol chloroform. This is the reason why you are getting the phenol chloroform. This is the reason why you are getting the phenol chloroform.

27:06

S… Speaker 2 (1 DNA Isolation and purification (mp3cut.net))

so you have chloroform and DNA

30:00

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

I think it's preferable to the wearable. It's just the wearable. Okay. Atomal alcohol is forming, sharpening, curries, salt, duralize, charges, precipitation, ethanol or isopropanol precipitation from the solution. 70% ethanol which is washed stored plus contamination for the wash day. And the heat buffer will stabilize and protect the DNA during the storage.

30:37

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

That is a Sleekah Column Based, which you have to keep in mind, is the personalization of the knowledge. You can learn how to learn how to learn how to learn how to learn how to learn how to learn how to learn how to learn how to learn how to learn how to learn how to learn how to learn how to learn how to learn how to learn how to learn

31:33

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

It depends upon the kit you are using at the time of isolation.

35:02

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

You can see it in the background. You can see it in the background. You can see it in the background.

36:09

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

DNA isolation and purification are a Vincent foundation technique in a molecular biology, enabling accurate downstream analyses such as PCR declines and cloning. Spinol chloroform extraction relies on the organic solvents of the EHF protein and separates the reagents, providing higher purity but involves the toxic reagents and longer processing plants.

36:35

S… Speaker 1 (1 DNA Isolation and purification (mp3cut.net))

Silica columbus method reversibly binds to the DNA to the silica membrane after rapid set and user friendly DNA purification is suitable for the most routine application. Each method has the distinct advantages and limitations choosing the appropriate technique depend upon the sample type required purity in the application. Understanding the chemical principles.

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