2026_Proteomics_L5_ENG_10-04-2026
Apr 10, 2026 09:29
· 54:23
· Italian
· Whisper Turbo
· 4 speakers
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Avete qualche dubbio riguardo ieri? Allora andiamo di espediti. Ultimo tipo di cromatografia
0:14
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
della quale volevo un attimo parlare di introdurvi perché viene molto utilizzata in proteome
0:21
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
ma soprattutto nella puliziazione di proteine ricombinanti.
1:14
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Allora, vi dicevo, l'ultima, devo farvi vedere, fa parte della categoria cromatografia
1:23
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
che è la finita e viene utilizzata moltissimo nella puliziazione di proteine ricombinanti.
1:32
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Per sintetizzare un certo tipo di proteine per endoscopi. Molte proteine vengono ad esempio
1:44
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
utilizzati anche in ambito terapia. E tra sisse, l'interesse, l'espresse, le vulture cellulari
2:03
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
esistono. Qual è il concetto però di tutta introdurla? È la cromatografia. Da un pulizia sintetizza la cromatografia
3:41
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
infizia. Quindi in realtà ci sono tanti che possono essere molto diversi.
4:03
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Possono, perché, nel caso che, possono essere più. Possono essere anche nel caso in cui, nel caso in cui il, la fase
4:38
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
è stata un'arma, la cromatografia di cui volevo parlarvi oggi.
4:52
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Grazie a tutti.
5:00
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
e darvi alcune informazioni riguardo alla fase stazionaria che vengono utilizzate in tutte le cromatografie per attività.
5:11
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Per denaro presenta una caratteristica che non abbiamo visto finora e che è proprio specifica della fase stazionaria utilizzata in questo caso per immunosimilio.
5:29
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Noi finora abbiamo sempre detto che la fase stazionaria in qualsiasi tipo di cromatografia è costituita dalla matrice polimerica che può essere di diverso tipo.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
L'attrice polimerica funge da supporto inerte, inerte fisicamente quindi fondiando specifici.
5:52
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
A seconda del tipo è possibile interagire con la cromatografia mediante specifiche interazioni deboli di cromatografie idrofobiche, river space cromatografie,
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
poi c'è anche la protezione ambiorionica.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Però questi erano i due punti, c'è un attacco ed è la nostra proteina che può essere qualsiasi cosa.
6:51
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Come dice una parola questo spazia dalla fase.
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Avete un'idea?
7:29
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Secondo voi, ragionandoci,
7:41
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Bravissima, perché vi ho detto prima che i legami possono essere elevati un contraservo,
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
possono essere proteine e quindi andare a legare direttamente il legame alla strazionale
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
potrebbe essere anche un problema, cioè potrebbe accadere in maniera efficiente ed efficace.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Perché? Quindi ci serve non solo il legame non sarebbe efficiente, ma magari la densità di legame così elevata,
8:47
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
ovvero per caso dell'antiquota è più facile per tutti, è di essere più massimo di un moleculomotico.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Quindi questo che cosa comporta? La fase strazionale è una bassa densità di legame più di una bassa di bagno.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Per far sì che la densità di legame ci permetta di avere una buona risoluzione delle strutture che hanno la futrice politica.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
assolutamente inarti nell'interagire con le nostre proteine, d'accordo?
10:06
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Quindi sono strutture, ve le faccio vedere qui, abbastanza semplici, nella maggior parte
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
dei casi sono delle catene di atomi di carbono, alcune possono contenere degli atomi di azoto,
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
degli atomi di ossigeno, ma prevalentemente, perché questi individui la possibilità di
10:43
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
che gli atomi di carbono possono dar luogo ad interazioni idrofobiche, ma lo sappiamo
10:48
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
che gli atomi idrofobici sono nel punto proteico, non sulla superficie delle nostre proteine,
10:54
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
mentre azoto, ossigeno possono dare interazioni. Ma allora, perché è possibile avere degli
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
azoti, degli ossigeni, perché in realtà, anche se sono presenti, anche se sono presenti,
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
non troviamo uno soltanto, se non sono presenti, non riescono ad interagire con gli altri, perché?
11:28
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Perché qui è importante anche considerare la lunghezza, ovvero ogni spaziatore ha una lunghezza,
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
che significa una composizione, un numero di atomi, tale per cui da creare, tale per cui
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
è possibile creare uno spazio, permettere al ligande di interagire e di legarsi in maniera
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
molto forte, ma di interagire con lo spazio. Soltanto possiamo dire che l'incontro e lo scarico
12:24
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
del ligande va a schermare, quindi è in proprio la dimensione, la lunghezza del spaziatore,
12:39
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
la quale non deve essere, almeno da un punto di vista teorico, interferire, quindi questo
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
non è una buona, perché ridurre la lunghezza dello spaziatore, la sua funzionalità, ovvero
13:09
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
lo spaziatore risulta essere troppo vicino alla superficie delle nostre sferette, può essere
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
una pronta e non riuscirebbe a legarsi, sarebbe delle sferette. Dall'altro, qui non avremo
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
spazio che contano 18 atomi, abbiamo visto essere il ligame della riba spesa, ma in questo caso
13:52
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
sarebbe troppo l'incontro, sarebbe troppo l'incontro, sarebbe troppo l'incontro di uscire
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
nello spazio stretto in gang, sarebbe in interagire, non solo la presa dello spaziatore, in realtà
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
questo ve l'ho spiegato, perché capiate la funzione, perché nel momento in cui c'erete,
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Selezionare una resina per una cromatografia per affinità andrete anche a valutare perché ci sono resine magari con lo stesso ligando ma con spacer diversi e lì quindi dovrete valutare voi quale potrebbe essere la migliore per la proteina di vostro interesse, d'accordo?
15:21
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Però in fase di scelta potrebbe essere utile sapere conoscere la funzione dello spacer per poi valutare quale resina sia la migliore nel vostro caso.
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Quindi anche in questo caso lo spacer è fondamentale, è imprescindibile, come ci vediamo, però dobbiamo fare in modo che lo spacer lo vedremo proprio a livello del picco.
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Quindi ragazzi, quando voi avete a livello cromatografico un po' molto allargato, se ad esempio lo speriamo con un siero da seccare, perché questo picco molto sbrovolato può essere dovuto al fatto che qualcosa viene luito un tempo troppo lungo.
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Potrebbe essere anche dovuto al fatto che non usate il programma di emizione corretto, che non usate un uso corretto ma troppo lento.
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Quindi la prima cosa da fare, prima di andare a pensare a fasi stazionali eccetera, cercare di cambiare le cose più semplici, il programma stesso.
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Se cambiando voi non migliorate in alcun modo, cosa significa in questo caso, probabilmente sotto quel picco nuovo viene luito una macchia.
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
In questo caso specifico, la fotografia di affinità potrebbe essere dovuta non tanto ad una scelta sbagliata del ligando, perché qui siete forse, la scelta della fase stazionale è più facile, perché sapendo che cosa purificare, voi scegliete fase stazionale con un determinato tipo di liste.
17:53
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Quindi aver scelto una fase stazionale con uno spacer sbagliato, con uno spacer che può andare ad interagire esso stesso, ok, con altre proteine.
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Quindi che cosa accade? In fase di eluizione, qui viene eluita non soltanto la proteina di interesse, cioè la proteina che ha interagito con il ligando, ma anche quelle proteine secondarie che non vi interessano, che possono aver interagito con lo spacer.
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Ok? Quindi di fatto questo vi fa capire che la separazione non è avvenuta con un'alta risoluzione.
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Questa resina non è selettiva nei confronti della vostra proteina di interesse, perciò mantenendo uguale il ligando posso fare di cambiare resina con uno spacer.
18:46
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Caro ragazzi, quindi quando qualcosa non va, cercate di cambiare a livello sperimentale le cose più semplici che potete cambiare.
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Poi eventualmente salite con il livello, diciamo così, ma prima cercate la soluzione più semplice.
19:11
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Bene, buongiorno, benvenuto.
19:16
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
So, now I change the language for...
19:21
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Hai sbagliato?
19:25
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Ok, don't worry, don't worry.
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Ok, before considering the immune activity, I just wanted to show in a brief way the experimental steps for the chromatography.
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Perché perché in questo caso c'è un'addizione che non abbiamo mai visto prima.
19:54
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
è un'addizione che non è un'addizione più semplice.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
also to show you the specific commission for the endodifference from the previous one okay so
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
what do you think about new addiction a step in the thromodrome do you recognize the new step
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
yesterday we have seen the first one the equilibration step let's see with a stutter buffer
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
is similar to the loading bar in this case okay but as a binding binding bar then there is a loading
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
step and normally after the loading step there is a dilution step in affinity chromatography there
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
is a one additional washing step and the washing step is performed with the same buffer that we
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
we have used for the equilibration step in other words the washing step is performed with the same buffer
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
so why is it present only in affinity chromatography we have in India affinity chromatography is for all
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
and in order to be sure to wash to eliminate all possible perform this additional step okay and so
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
after this working step we are sure that inside our column we can find only one sample sample okay
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
well and what about the illusion step also in this case the illusion step is performed with a different
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the mobile frame formed by two people the first one is devoted to hydrolyze the weak interactions
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
first one performed formed between legal and the third another one of the second structure is
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
viewed by the possibility a sort of that is characterized by very high affinity for such a competitor is usually
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
which is a decrease and at a very high and so the high concentration and the higher there are different
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
strategies as a regard to the possibility to hydrolyze the process the condition of the relevant
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
buffer will be specific in order to for example change the ph all that
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Weak interactions are viewed by ionic interactions, abandoning the changes of modes are possible to change modes.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
And if we change the chart, change is idealized.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
In fact, the use of competitor solutions is normally chosen as such.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Because by using a competitor, you don't change the economy inside the earth.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Why? If you wanted to hydrolyze in a different direction, you have to change something in your throat.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
In fact, you can change the pH or you can introduce high concentration of salt in order to increase the ionic strength.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
In fact, you can change the ions with the result of the length of heat also with your proteins.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Or you can change the polarity, introducing an organic solvent.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
In fact, you know that may make no problems.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
And if you choose.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
So we can say that normally, we prefer to use the strategy of the content.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
In this condition, the bound process,
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
doesn't change their charge balance.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Because the vacuum is repaired by the company.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
So the composition for us is reliable and reproducible.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
The most used type of elution is the elution through a specific competitor.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Okay, and then again the equilibrate.
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Okay.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
I wanted to show you only one example of affinity chromatography.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Where our stationary phase is characterized by the presence of antibodies.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
The choice depends on the domain.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
The research of sport.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Because, for example, if you wanted to characterize a particular group, a particular family, choose
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
a stationary phase with a polyclone.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
But if you are working in a hospital and you want to run out to human disease.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
And you need to demonstrate the presence of a particular phase with a monoclonal.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
In this case, you are sure that possible pathological one.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
because, of course, the course...
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
So, of course, the course...
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
And you need to be able to convey an answer.
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
The course of course because manyammers...
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
In questo caso, ci sono delle specie di immuno affiniti cromotografi.
30:06
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Un altro importante aspecto che abbiamo parlato prima,
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
ma che è molto importante con tutti gli affiniti cromotografi,
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
è che in caso di affiniti cromotografi,
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
ci sono dei poli, come gli affiniti cromotografi.
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
In affiniti cromotografi,
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
ci sono delle specie di immuno affiniti cromotografi,
30:47
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
che sono stati cromotografi cromotografi,
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
che sono stati cromotografi cromotografi,
30:57
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
che sono stati cromotografi,
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
perché in questo caso,
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
si è stato fatto,
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
ci sono stati cromotografi,
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
quindi ci sono dei poli di immuno affiniti sui cromotografi,
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
quindi per questo motivo,
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
ci abbiamo bisogno di aumentare il profilo di dimensione.
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Il profilo di immuno affiniti cromotografi,
31:35
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
poi ci siamo stati cromotografi,
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Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
e quindi ci sono stati cromotografi,
31:47
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
in meno che i quindi,
31:49
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
fati cromotografi cromotografi cromotografi,
31:52
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
quindi, in questo motivo,
31:53
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
la maggioranza della stazione dei fasi della stazione dei fasi della stazione dei fasi
32:07
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
di supporto.
32:09
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Ok?
32:11
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Ehm...
32:12
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Well...
32:13
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
So...
32:14
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
I wanted to considerate un aspect
32:20
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
di stazione dei fasi della stazione dei fasi della stazione dei fasi di immunofidio.
32:26
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
So...
32:26
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
We'll realize that you have macrobolus...
32:32
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Macrobolus beads.
32:34
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Se io anche...
33:12
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Stavamo...
33:12
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Un nome la tiene
33:21
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
che altrimenti si incasca.
33:28
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Ci siamo?
33:29
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
C'è il nome?
33:31
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
No.
33:33
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
No, no.
33:34
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Ok.
33:34
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
E' un altro particolare aspect
33:36
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
for stazione dei fasi in immunofidio
33:41
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
is...
33:47
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
to the stazione dei fasi.
33:50
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
because the structure bind the antibodies called the divide out
34:05
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
that are related to the resolution.
34:10
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
In the first one we have to proceed to obtain
34:14
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
the solutions for stazione dei fasi
34:18
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
with quite a cheaper cost.
34:23
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
In the other case, why?
34:33
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
It would be possible to recognize and to divide.
34:39
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Because the antibodies could be bound to the stazione dei fasi
34:44
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
all through random chemicals.
34:52
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
So...
34:54
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
which one is the...
34:59
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
the random...
35:06
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Sì, cosa? Ok, direttionalità.
35:20
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
So, let's try to understand it together.
35:25
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
So, what do you think about the binding capacity for both,
35:32
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
that means the possibility for anti-barbic to the bonanza and to bind,
35:42
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the binding capacity could be the same in random,
35:51
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
but in the random order, for this reason, we have a higher solution.
36:07
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Our immuno chain becomes dromatosa.
36:19
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Anti-barbic attaches through a direction and a reaction,
36:28
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the random attachment induces.
36:41
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
The first one is random, so you can, the antibody can attach to anything,
36:47
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
maybe it's compacted with it.
36:50
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
Second case, it's unreactive, correct.
36:56
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Great. And, considering today, anti-barbic,
37:01
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
remember the two-band to recognize and the two-band proteins,
37:12
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
they are active, but the type of sequences on the protein,
37:22
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
that is, in the corner.
37:24
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
My question is, do you remember the region in the antibody
37:29
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
devoted to recognize and to bind the protein?
37:38
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
So, no question. Yeah, it's correct.
37:42
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Ok, there are two important regions in all antibodies,
37:48
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
to bind the flow, they are called wild,
37:56
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
it's only people who can select the protein.
38:01
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
So, the direction involved in the antibody,
38:18
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the direction, is that,
39:03
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
change through a position.
39:10
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Do you understand why to understand,
39:26
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
we have seen a difference that the direction of the band
39:34
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
to grab it in order to increase the bandwidth,
39:38
S…
Speaker 3 (2026_Proteomics_L5_ENG_10-04-2026)
the high bandwidth of the band,
39:44
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Grazie per la visione!
40:05
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
and so the polymericane in random chemicals, and so for the chemical etat, anablarose B contains a lot of molecules,
40:38
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
but some of them are constant regions, and so they are free, recognized, and infertile.
40:54
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Is this kind of antibody to bind?
41:00
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
For this reason, the binding capacity in a random chemical attachment is lower than the ligand density involved in a random chemical molecule interact with homograms
41:39
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
are bound to the variable root to prevent the interaction with our protein.
41:49
S…
Speaker 3 (2026_Proteomics_L5_ENG_10-04-2026)
Ok? Chiaro ragazzi?
41:51
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
It's not important to remember that this is the scheme of random chemicals.
42:10
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
In this case, the agarose beads, the polymeric beads, are functionalized with a very reactive
42:23
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
that is able to recognize and to bind the amino groups present in our antibodies.
42:42
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
I mean, ok, this is the reaction, this is the amino group present in our antibodies,
42:52
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
and this is the covalent, stronger chemical binding between these and others.
43:05
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Ok? But of course, if leasing or vesicoline are released on the top, the antibody is to the stationary phase through constant region,
43:25
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
and so the variable regions are free to recognize and to interact.
43:32
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
But if such basic antibodies are present on the variable region, in this case, the antibody is attached through the variable region.
43:44
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
And the constant region is free for protein interaction.
43:52
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
And we know that it's impossible for the constant region to recognize and to bind.
43:59
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
For this reason, such binding is not useful for ours.
44:07
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
For this reason, this kind of are characterized, could be characterized by the same region density,
44:20
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the same number of regions, the same number of atoms, or all around,
44:28
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
creates a locus to recognize and bind.
44:46
S…
Speaker 4 (2026_Proteomics_L5_ENG_10-04-2026)
Ok.
44:47
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
We have some surface that is suitable for us,
44:51
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
and the same number of atoms.
44:56
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
So the random...
45:11
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
non so disegnare
45:15
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
this is our beat
45:18
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
this is the antibody
45:22
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
the variable region
45:24
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
devoted to bind
45:26
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
to recognize and to bind the protein
45:29
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
are this one
45:30
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
this is the constant region
45:34
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
if the random attachment
45:37
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
occurs in this direction
45:40
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
this is the space in this direction
45:43
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
in the constant region
45:46
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
the antibody may interfere
45:50
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
with our protein
45:52
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
to recognize and to bind
45:55
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
but in the case that
45:57
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
this random attachment
46:00
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
occurs on the variable region
46:08
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
we not only reduce the possibility
46:11
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
to recognize the proteins
46:13
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
because in this case
46:15
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
they are very close to the beads
46:17
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
it's important for my protein
46:19
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
to interact with the antibody
46:22
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
so we have two molecules of antibodies
46:26
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
but only one
46:27
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
it's active and selective
46:29
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
for the recognition of our protein
46:34
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the second portion is not suitable for us
46:37
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
no
46:37
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
no
46:38
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
but maybe
46:39
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
this kind could be present
46:42
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
in this kind of
46:43
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
stationary phase
46:45
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
for this reason
46:45
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
they are cheaper
46:46
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
okay
46:48
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
but it's impossible
46:52
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
to prevent the second phase
46:54
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
during the preparation
46:55
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
okay
46:57
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
if you wanted to eliminate
47:01
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
such a condition
47:04
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
you should buy the stationary phase
47:09
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
prepared through a directional attachment
47:12
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
in the directional attachment
47:14
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
we use a particular spacer
47:17
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
called protein A or G
47:20
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
they are little protein
47:23
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
the proteins are basically
47:28
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
the constant region
47:34
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
and so
47:36
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
we are sure
47:37
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
that
47:38
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
through
47:39
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
protein A or G
47:42
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
our antibodies
47:45
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
are bound
47:47
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
only through
47:48
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
constant region
47:50
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
and so
47:51
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
all variable regions
47:53
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
are free
47:54
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
to recognize
47:55
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
and interact
47:57
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
our protein
47:58
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
okay
47:59
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
such
48:01
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
interaction
48:02
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
is stabilized
48:03
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
by the addiction
48:05
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
of a cross-linker reaction
48:08
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
okay
48:08
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
just to stabilize
48:10
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
the binding
48:12
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
between
48:13
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
protein A or G
48:15
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
with our antibody
48:16
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
we can say
48:17
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
that the protein A
48:19
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
is useful
48:20
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
in order to
48:21
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
select
48:23
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
basically
48:30
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
in this case
48:31
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
we can say
48:32
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
that
48:33
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
the ligand
48:35
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the density
48:38
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
strike the related binding
48:41
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
because
48:43
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
all
48:44
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
bound
48:45
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
and interact
48:51
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
our protein
48:52
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
okay
48:53
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
of course
48:54
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
this kind of
48:55
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
stationary phase
48:56
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
are
48:58
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
quite
48:59
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
expensive
49:00
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
the choice
49:17
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
between
49:18
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
the antibody
49:21
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
and
49:22
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the
49:23
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
yeah
49:26
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
I don't get
49:28
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
how
49:31
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
whichever
49:31
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
one
49:33
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
would
49:34
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
be
49:35
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
because
49:35
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
I remember
49:37
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
that
49:37
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the last
49:37
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
part
49:38
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
of
49:38
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the
49:39
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
antimicrobial
49:40
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the
49:42
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
basic
49:42
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
part
49:49
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the
49:50
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
basic
49:52
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the
49:52
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
basic
49:52
S…
Speaker 2 (2026_Proteomics_L5_ENG_10-04-2026)
max
49:53
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
and
49:54
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
in
49:55
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
in
49:55
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
realtà
49:55
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the
49:58
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
important
49:58
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Grazie a tutti
50:00
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
di aminoacidi basici.
50:02
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Ah, non è la...
50:03
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
No, no, non è l'anio terminale.
50:06
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
No, no, no, aminoacidi basici.
50:08
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Quando parlo di aminoacidi basici
50:10
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
parlo degli ammone sui residui.
50:13
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Ok, no, no, no.
50:17
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Ok, so,
50:19
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
I wanted to conclude
50:21
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
by considering
50:24
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
briefly
50:24
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the experimental
50:26
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
ok, so we decided to buy
50:30
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the most expensive
50:32
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
stationary phase.
50:34
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
Ok, we are
50:35
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
bravo, we are rich
50:38
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
in reality
50:40
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
but we dream
50:42
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
ok, because
50:47
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
we wanted to have
50:49
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the maximum biocapacity
50:51
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
and the maximum selectivity
50:53
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
for one protein.
50:55
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
So, we equilibrate
50:57
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
our column with
51:00
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the buffer that is
51:02
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the same on which
51:04
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
our proteins are stored.
51:07
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
then we load
51:09
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
our phone.
51:11
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
After this loading step
51:13
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
we wash
51:16
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
to favor the elimination
51:17
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
of unbounded
51:19
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
and then
51:21
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
we start with
51:22
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the elution.
51:24
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
We normally
51:25
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
use
51:26
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
a solution
51:27
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
containing
51:28
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
a competitor
51:29
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
agent.
51:30
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
The competitor
51:31
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
is normally
51:33
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
present
51:36
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
together
51:40
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
it is easier
51:41
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
for us
51:42
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
to obtain
51:43
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the most
51:45
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
reproducible
51:47
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
condition
51:47
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
of our
51:50
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
experiment.
51:52
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
But
51:55
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
to use
51:59
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
a mobile
52:00
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
fader
52:01
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
to
52:02
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
hydrolyze
52:08
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
our
52:09
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
proteins
52:09
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
and
52:10
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
our
52:11
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
proteins.
52:12
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
This
52:12
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
kind of
52:13
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
interaction
52:14
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
is mostly
52:16
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
vulnerable
52:17
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
as
52:19
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
hydroton.
52:21
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
and so
52:21
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
in order
52:23
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
to hydrolyze
52:27
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
would be
52:28
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
to change.
52:32
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
This is an
52:33
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
example.
52:35
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
I mean
52:36
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the previous
52:37
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
step
52:37
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the loading
52:38
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
and the washing
52:39
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
steps
52:39
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
are performed
52:41
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
and all
52:42
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
you know
52:45
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
that
52:45
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
proteins
52:47
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
well
52:47
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
solidized
52:48
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
are
52:49
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
very stable
52:50
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
and the possibility
52:53
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
could be
52:54
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
to reduce
52:54
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the pH.
52:56
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
In this
52:57
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
case
52:58
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
all
52:58
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
bundle
52:59
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
does
52:59
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
interact
53:07
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
I don't
53:08
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
like
53:08
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the
53:11
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
pathogen
53:11
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
because
53:12
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
I mean
53:14
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the difference
53:15
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
of the pH
53:16
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
is very
53:19
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
high.
53:21
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
This
53:21
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
high
53:21
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
difference
53:27
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
to a
53:27
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
youth
53:35
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
to be
53:37
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
done
53:41
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
favor
53:42
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the
53:43
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
flexibitation
53:44
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
of the
53:45
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
proteins
53:45
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
inside
53:46
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the
53:46
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
column
53:46
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
means
53:47
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the
53:48
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
damage
53:50
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
of it.
53:52
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
The
53:53
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
columns
53:53
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
are
53:53
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
very
53:54
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
expensive.
53:55
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
We
53:55
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
are
53:55
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
rich
53:55
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
but
53:56
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
not
53:56
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
so
53:56
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
rich.
54:00
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
it's
54:01
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
important
54:01
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
to
54:02
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
know
54:02
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
that
54:02
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
this
54:03
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
is
54:03
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
a
54:03
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
possibility
54:04
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
from
54:04
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
a
54:05
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
theoretical
54:05
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
point
54:05
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
of
54:06
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
view
54:06
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
or
54:08
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
experimental
54:09
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
point
54:09
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
of
54:09
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
view
54:10
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
all
54:10
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
of
54:10
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the
54:10
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
simulation
54:11
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
is
54:11
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
compared
54:12
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Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
to
54:12
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
the
54:12
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
solution.
54:17
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
I
54:18
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
think
54:18
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
that
54:18
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
that's
54:19
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
all.
54:20
S…
Speaker 1 (2026_Proteomics_L5_ENG_10-04-2026)
See?
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