GEN3001_Workshop 3

Hökmdar

• Speaker 1 0:15
• Speaker 2 1:20
• Speaker 3 1:28
• Speaker 4 1:48
• Speaker 5 6:33
• Speaker 6 57:38
• Speaker 7 1:00:27
• Speaker 8 1:14:41

1. 0:15

   Bölmə 1: Thank you. 33s · Speaker 1

   Thank you. Thank you.

2. 1:20

   Bölmə 2: okay um hi morning um You all know where to get your materials, right? 301s · Speaker 2

   okay um hi morning um You all know where to get your materials, right? Okay, very good. We have two sessions today, correct? Later on, I will upload the workshop number four. But maybe we might not... Okay, we'll see how it goes. We might n…

3. 6:21

   Bölmə 3: the age group. 265s · Speaker 2

   the age group. Otherwise, it will be a very biased study. You also need to factor in gender. Okay, because you cannot only select for one gender and ignore the other. Okay, and then the last one, which is also quite important, will be ethni…

4. 10:47

   Bölmə 4: okay, I have variants 1, 2, 3. 90s · Speaker 3

   okay, I have variants 1, 2, 3. All these are the differences I get. between my case and my control. And I say that these differences are what drives type 2 diabetes. You cannot say this. What you can say is that they are probably related to…

5. 12:17

   Bölmə 5: Okay, so what is pedigree studies? 279s · Speaker 1

   Okay, so what is pedigree studies? You study people who are related by blood, right, within a family. Okay, and then you can nail down to which one is the one that caused the disease in that family. Okay, so this is GWAS. And then if we loo…

6. 16:57

   Bölmə 6: right? And then that will give you an indication about diabetes risk profile. 301s · Speaker 3

   right? And then that will give you an indication about diabetes risk profile. But you can also pick up profile risk for other type of diseases that are associated with type 2 diabetes, for example. So maybe things like cardiovascular risk, …

7. 21:58

   Bölmə 7: generation of offspring. 300s · Speaker 2

   generation of offspring. You have to really look at the genotype itself because it is the genotype that helps to manifest the phenotype. Okay, so then that will sum up 3 .1. Okay, in 3 .2 here, we are going to have a little bit of a mouse m…

8. 26:59

   Bölmə 8: I will breed more of this minus minus mice. 300s · Speaker 2

   I will breed more of this minus minus mice. And then I can study what kind of phenotype it gives me. Okay, so slide 12, right, basically telling you that you can actually use the mice at different levels so you can create. different types o…

9. 31:59

   Bölmə 9: storage wise it's not a big problem because you can house them in different tanks and you can put them inside or slot them inside those shelves okay so low cost definitely compared to mice okay because the mice themselves are charged by per animal and they are charged by per housing unit that they are in, and they are also charged by the person taking care of them. 281s · Speaker 2

   storage wise it's not a big problem because you can house them in different tanks and you can put them inside or slot them inside those shelves okay so low cost definitely compared to mice okay because the mice themselves are charged by per…

10. 36:41

    Bölmə 10: Okay, so the slide here is just a side -by -side comparison of your transient versus stable, provided that you already know what each and every one of these techniques does. 78s · Speaker 4

    Okay, so the slide here is just a side -by -side comparison of your transient versus stable, provided that you already know what each and every one of these techniques does. Okay, so go back and refer to the extra notes I've given you. Now,…

11. 37:59

    Bölmə 11: right? So the ones that are left without the permanency will be the first two, okay? 133s · Speaker 5

    right? So the ones that are left without the permanency will be the first two, okay? So morpholinos themselves are what we call knockdowns rather than knockout, okay? So here you reduce expression of the gene. It's a downregulation. Now, it…

12. 40:12

    Bölmə 12: Okay, so if you recall from Gen 2, plasmids are able to independently replicate by themselves because they have their own origin of replication. 300s · Speaker 3

    Okay, so if you recall from Gen 2, plasmids are able to independently replicate by themselves because they have their own origin of replication. Okay, so it doesn't really require any host for it to replicate. Okay, so not very often used f…

13. 45:13

    Bölmə 13: Different from us where we develop the germ layers. 173s · Speaker 2

    Different from us where we develop the germ layers. Okay, so gastrulation for the zebrafish means that there is a polarity. They know where is the anterior, where is the posterior, which one becomes the head structure, which is the anterior…

14. 48:07

    Bölmə 14: Okay. Now, to answer this question, you will then have to use a reverse genetics because you already know the gene involved. 301s · Speaker 5

    Okay. Now, to answer this question, you will then have to use a reverse genetics because you already know the gene involved. You are trying to study the phenotype, which is about the jaw development. Okay. So, your hypothesis for this quest…

15. 53:08

    Bölmə 15: And of course, the embryo will eventually not live, right? 202s · Speaker 2

    And of course, the embryo will eventually not live, right? Because it's quite lethal at that early part of the development. Okay, so now the question here is, if you want to see this kind of structural change, and if you do something that i…

16. 57:38

    Bölmə 16: That's crazy. 147s · Speaker 6

    That's crazy. I think we'll stop for it. What is it? That's the bridge In this class, there is a lot of check these ones. I can't believe that's even though I... I didn't think it's almost lost.

17. 1:00:27

    Bölmə 17: Oh, it's cool. 128s · Speaker 7

    Oh, it's cool. Yeah, it's cool. I see it. I see it. Should we find out? Yeah, should we find out? Unless outside you see 2,000. They'll be right. We all keep thinking. Wait, sorry. That's a great thing. It's not really perfect, okay, hello.…

18. 1:02:35

    Bölmə 18: much. I just swam off. 133s · Speaker 1

    much. I just swam off. I don't want to smoke. I don't want to smoke. I don't want to smoke. I don't want to smoke. Hey, everyone is back, right? Can you guys open up your worksheet? Okay, we will have to split this with four groups, like wh…

19. 1:04:48

    Bölmə 19: or maybe you can join this group. 76s · Speaker 3

    or maybe you can join this group. what was i thinking okay then okay can you pretend that i didn't upload the answer Thank you.

20. 1:06:22

    Bölmə 20: Thank What? 323s · Speaker 1

    Thank What? What? Did somebody call me? Oh okay. okay let's just click the questions um people at the best Okay, Carolyn, your group, can you do question one? Okay, and then you guys, can you take question two? Okay, you guys take question …

21. 1:12:01

    Bölmə 21: So you're just going to tell you how the gene can write, okay, or it's just the smallest gene to do a small job. 158s · Speaker 6

    So you're just going to tell you how the gene can write, okay, or it's just the smallest gene to do a small job. And then let's see if the last question Oh, no. No. Monash, you never sleep? No. Monash, don't go. Oh. Oh. Oh. Also having this…

22. 1:14:41

    Bölmə 22: Thank you. · Speaker 8

    Thank you. for the email

23. 1:46:16

    Bölmə 23: you're putting like any like styrofoam based to like oh it really helped my girlfriend Okay, before we get into the first part of the class, you can take a look at the answers that your friends have provided. 128s · Speaker 4

    you're putting like any like styrofoam based to like oh it really helped my girlfriend Okay, before we get into the first part of the class, you can take a look at the answers that your friends have provided. I checked, I roughly can see th…

24. 1:48:24

    Bölmə 24: Okay, because they are doing this as an in vitro essay, so you can do this with a controlled level of dosages. 41s · Speaker 1

    Okay, because they are doing this as an in vitro essay, so you can do this with a controlled level of dosages. So yeah, then this side would be the question, the last question, question 4. So okay, I don't have any problem with ABC. Just th…